JenKem Commercial Scale PEG productionJenKem Technology provides high quality activated multi-arm polyethylene glycol derivatives (PEGs) with high purity and low polydispersity.

JenKem Technology’s multi-arm PEG derivatives can be cross-linked into hydrogels. PEG hydrogels have a variety of applications in medical devices and regenerative medicine, and are especially of interest for controlled release of drugs, for 3D cell culture, and for wound sealing and healing [1].

JenKem Technology’s multi-arm star PEGs are synthesized by ethoxylation of tripentaerythritol (8ARM(TP) PEG), hexaglycerol (8ARM PEG), dipentaerythritol (6ARM PEG), pentaerythritol (4ARM PEG), or glycerol (3ARM PEG). The number of ethylene oxide units in the PEG chain may not be equal for all arms. The total molecular weight reported for the JenKem multi-arm PEGs is the sum of the molecular weights of the PEG chains on each arm. 8ARM(TP)-PEGs with tripentaerythritol core have a higher purity as evidenced by MALDI compared to the generic 8ARM-PEGs with a hexaglycerin core.

8ARM TP CORE ADVANTAGES

Multi-arm star PEG products with molecular weights and functional groups not listed in our online catalog may be available by custom synthesis. Please inquire at tech@jenkemusa.com about pricing and availability.

JenKem Technology provides GMP grade PEG derivatives and bulk orders via custom synthesis, offering the opportunity to match customers’ special quality requirements. JenKem Technology is capable of development and synthesis of a wide range of GMP PEG derivatives starting at 200g up to 40 kg or greater batches, under ISO 9001 and ISO 13485 certified quality management system, following ICH Q7A guidelines. For inquiries on cGMP production of PEG derivatives please contact us at tech@jenkemusa.com.

For global distribution, please visit link. Please click the buttons below to order directly from JenKem Technology:

3ARM PEG DERIVATIVES
3ARM PEG PRODUCT SUBSTITUTION REACTIVITY DETAILS
≥ 95% 3arm PEG Amine. Hydrogel PEG. Amine group binds to carboxylic group (-COOH) or other amine reactive chemical groups
4ARM PEG DERIVATIVES
4ARM PEG PRODUCT SUBSTITUTION REACTIVITY DETAILS
≥ 95% 4arm PEG Amine, Free Amine. Hydrogel PEG. Amine group binds to carboxylic group (-COOH) or other amine reactive chemical groups [2, 6, 8]
≥ 95% 4arm PEG Amine, HCl Salt. Hydrogel PEG. Amine group binds to carboxylic group (-COOH) or other amine reactive chemical groups [3-5]
≥ 95% 4arm PEG Carboxyl (4arm PEG Acetic Acid, 4arm-COOH, 4arm-CM). Hydrogel PEG. Carboxyl group binds to amino or other acid reactive chemical groups [7]
≥ 95% 4arm PEG SCM (4arm PEG NHS Ester). Hydrogel PEG. This is the activated form of 4ARM-COOH. [8, 9]
≥ 95% 4arm PEG Succinimidyl Glutaramide. Hydrogel PEG. SGA has a longer hydrolysis half-life compared with SCM [23].
≥ 95% 4arm PEG Nitrophenyl Carbonate. Hydrogel PEG. Carbonate linker between PEG and NHS ester; the reaction with amine groups releases p-nitrophenol which can be easily traced by UV spectroscopy.
> 90% 4arm PEG Succinimidyl Carbonate. Hydrogel PEG. Carbonate linker between PEG and NHS ester; longer hydrolysis half-life compared with SCM
> 90% 4arm PEG Maleimide. Hydrogel PEG. Maleimide is selective for thiol groups and reacts at pH 5.0-6.5. [24-27]
≥ 95% 4arm PEG Acrylate. Hydrogel PEG. Used in vinyl polymerization or co-polymerization [10]
> 90% 4arm PEG Thiol. Hydrogel PEG. Selective for thiol groups under mild reaction conditions [11]
> 90% 4arm PEG Vinylsulfone. Hydrogel PEG. VS binds free thiol groups in aqueous buffer between pH 6.5~8.5 at room temperature [12]
≥ 95% 4arm PEG Succinimidyl Succinate. Hydrogel PEG. Cleavable PEG linker. The ester linker between PEG and NHS ester enables the feature of “degradable hydrogel”. [28]
≥ 95% 4arm PEG Succinimidyl Glutarate. Hydrogel PEG. Cleavable PEG linker. The ester linker between PEG and NHS ester enables the feature of “degradable hydrogel” [13].
> 90% 4arm PEG Isocianate. Hydrogel PEG. NCO group is useful for coupling hydroxyl through a stable urethane linker
≥ 95% 4arm PEG Azide. Hydrogel PEG. Azide group reacts with alkynes in aqueous solution catalyzed by copper [14]
4ARM PEG RAW MATERIALS
4ARM PEG RAW MATERIALS MAIN PEAK FRACTION BY GPC POLYDISPERSITY BY GPC
≥ 95% ≤ 1.05
6ARM PEG DERIVATIVES
6ARM PEG PRODUCT SUBSTITUTION REACTIVITY DETAILS
≥ 95% 6arm PEG Amine. Crosslinks into hydrogels. Amine group reacts with carboxylic group (-COOH) or other amine reactive chemical groups [15]
6ARM PEG RAW MATERIALS
6ARM PEG RAW MATERIALS MAIN PEAK FRACTION BY GPC POLYDISPERSITY BY GPC
≥ 95% ≤ 1.08
 8ARM PEG DERIVATIVES WITH TRIPENTAERYTHRITOL CORE
8ARM PEG PRODUCT SUBSTITUTION REACTIVITY DETAILS
≥ 95% 8arm PEG Amine. Crosslinks into hydrogels. Amine group reacts with carboxylic group (-COOH) or other amine reactive chemical groups . 8ARM(TP)-PEG with tripentaerythritol core has a lower polydispersity and higher molecular weight accuracy compared with the generic 8ARM-PEG with a hexaglycerol core.[16]
≥ 95% 8arm PEG Carboxyl. Crosslinks into hydrogels. Carboxyl group reacts with amino or other acid reactive chemical groups. 8ARM(TP)-PEG with tripentaerythritol core has a lower polydispersity and higher molecular weight accuracy compared with the generic 8ARM-PEG with a hexaglycerol core. [29-31]
> 90% 8arm PEG Maleimide. Crosslinks into hydrogels. MAL is selective for thiol groups on cystein side chains; reacts at pH 5.0-6.5. 8ARM(TP)-PEG with tripentaerythritol core has a lower polydispersity and higher molecular weight accuracy compared with the generic 8ARM-PEG with a hexaglycerin core. [32]
≥ 95% 8arm PEG Acrylate. Crosslinks into hydrogels. Used in vinyl polymerization or co-polymerization. 8ARM(TP)-PEG with tripentaerythritol core has a lower polydispersity and higher molecular weight accuracy compared with the generic 8ARM-PEG with a hexaglycerol core. [33]
> 90% 8arm PEG Thiol. Crosslinks into hydrogels. Selective for thiol groups under mild reaction conditions. 8ARM(TP)-PEG with tripentaerythritol core has a lower polydispersity and higher molecular weight accuracy compared with the generic 8ARM-PEG with a hexaglycerol core. [34, 35]
> 90% 8arm PEG Vinylsulfone. Crosslinks into hydrogels. VS reacts with free thiol groups in aqueous buffer between pH 6.5~8.5 at room temperature. 8ARM(TP)-PEG with tripentaerythritol core has a lower polydispersity and higher molecular weight accuracy compared with the generic 8ARM-PEG with a hexaglycerol core. [36-38]
≥ 95% 8arm PEG Succinimidyl Succinate. Crosslinks into hydrogels. Cleavable PEG linker. The ester linker between PEG and NHS ester facilitates the formation of degradable hydrogel. 8ARM(TP)-PEG with tripentaerythritol core has a lower polydispersity and higher molecular weight accuracy compared with the generic 8ARM-PEG with a hexaglycerol core. [39]
≥ 95% 8arm PEG Succinimidyl Glutarate. Crosslinks into hydrogels. Cleavable PEG linker. The ester linker between PEG and NHS ester facilitates the formation of degradable hydrogel. 8ARM(TP)-PEG with tripentaerythritol core has a lower polydispersity and higher molecular weight accuracy compared with the generic 8ARM-PEG with a hexaglycerol core. [40]
> 85% 8arm PEG Norbornene. Crosslinks into hydrogels. Norbornene NB PEGs are suitable for copper-free click chemistry reactions with tetrazines and for thiol-ene click reactions with thiols. 8ARM(TP)-PEG with tripentaerythritol core has a lower polydispersity and higher molecular weight accuracy compared with the generic 8ARM-PEG with a hexaglycerol core. [41-44]
8ARM PEG DERIVATIVES WITH HEXAGLYCEROL CORE
8ARM PEG PRODUCT SUBSTITUTION REACTIVITY DETAILS
≥ 95% 8arm PEG Amine. Crosslinks into hydrogels. Amine group reacts with carboxylic group (-COOH) or other amine reactive chemical groups [17-18]
≥ 95% 8arm PEG Carboxyl. Crosslinks into hydrogels. Carboxyl group binds amino or other acid reactive chemical groups [16]
> 90% 8arm PEG Maleimide. Crosslinks into hydrogels. MAL group is selective for thiol groups; reacts at pH 5.0-6.5.[19, 46].
≥ 95% 8arm PEG Acrylate. Crosslinks into hydrogels. Used in vinyl polymerization or co-polymerization [20]
> 90% 8arm PEG Thiol. Crosslinks into hydrogels. Selective for thiol groups under mild reaction conditions [21]
≥ 95% 8arm PEG Succinimidyl Succinate. Crosslinks into hydrogels. Cleavable PEG linker. The ester linker between PEG and NHS ester facilitates the formation of degradable hydrogel. Binds to amino group of lysine(s) or N-terminal amines [39, 45]
≥ 95% 8arm PEG Succinimidyl Glutarate. Crosslinks into hydrogels. Cleavable PEG linker. The ester linker between PEG and NHS ester facilitates the formation of degradable hydrogel [22]
8ARM PEG RAW MATERIALS
8ARM PEG RAW MATERIALS MAIN PEAK FRACTION BY GPC POLYDISPERSITY BY GPC
≥ 95%(> 90% for MW 40000 Da TP core) ≤ 1.08 (TP core) (≤ 1.12 (hexaglycerol core))

For information on heterobifunctional PEGs please visit:

MULTIARM HETEROBIFUNCTIONAL PEGs
REFERENCES
  1. Hutanu, D., et al., Recent Applications of Polyethylene Glycols (PEGs) and PEG Derivatives. Mod Chem appl, 2014, 2(132).
  2. Zhan, H., et al., A Hybrid Peptide Amphiphile Fiber PEG Hydrogel Matrix for 3D Cell Culture, Advanced Functional Materials, 2019.
  3. Bizeau, J., et al., Synthesis and characterization of hyaluronic acid coated manganese dioxide microparticles that act as ROS scavengers, Colloids and Surfaces B: Biointerfaces, 2017, 159, P. 30-38.
  4. Li Y, et al., Water-dispersible graphene/amphiphilic pyrene derivative nanocomposite: High AuNPs loading capacity for CEA electrochemical immunosensing, Sensors and Actuators B: Chemical, 2017.
  5. Aliperta, R., et al., Cryogel-supported stem cell factory for customized sustained release of bispecific antibodies for cancer immunotherapy, Scientific Reports, 2017, 7.
  6. Bachmann, D., et al., Retargeting of UniCAR T cells with an in vivo synthesized target module directed against CD19 positive tumor cells, Oncotarget, 2018, 9(7), p.7487.
  7. Dai, L., et al., Self-assembled targeted folate-conjugated eight-arm-polyethylene glycol–betulinic acid nanoparticles for co-delivery of anticancer drugs, J. Mater. Chem. B, 2015, 3, 3754-3766.
  8. Mou, C., et al., Electrochemical-mediated gelation of catechol-bearing hydrogels based on multimodal crosslinking, Journal of Materials Chemistry B., 2019.
  9. Ding, Y., et al., Biomimetic soft fibrous hydrogels for contractile and pharmacologically responsive smooth muscle, Acta biomaterialia, 2018.
  10. Casey, J., et al., 3D hydrogel-based microwell arrays as a tumor microenvironment model to study breast cancer growth, Biomedical Materials, 2017, 12(2):025009.
  11. Dos Santos, B.P., et al., Production, purification and characterization of an elastin-like polypeptide containing the Ile-Lys-Val-Ala-Val (IKVAV) peptide for tissue engineering applications, Journal of biotechnology, 2019, 298:35-44.
  12. Van den Broeck, L., et al., Cytocompatible carbon nanotube reinforced polyethylene glycol composite hydrogels for tissue engineering, Materials Science and Engineering: C, 2019.
  13. Delgado, L., et al., Collagen cross-linking–Biophysical, biochemical and biological response analysis. Tissue Engineering, 2017.
  14. DeForest, C.A., E.A. Sims, and K.S. Anseth, Peptide-Functionalized Click Hydrogels with Independently Tunable Mechanics and Chemical Functionality for 3D Cell Culture. Chemistry of Materials, 2010, 22(16): p. 4783-4790.
  15. Chong, Y., et al., The in vitro and in vivo toxicity of graphene quantum dots. Biomaterials, 2014, 35(19): p. 5041-5048.
  16. Li, C., et al., An efficient prodrug-based nanoscale delivery platform constructed by water soluble eight-arm-polyethylene glycol-diosgenin conjugate, Materials Science and Engineering: C, 2019, 98:153-60.
  17. Schneider, M.C., et al., An In Vitro and In Vivo Comparison of Cartilage Growth in Chondrocyte-Laden Matrix Metalloproteinase-Sensitive Poly (Ethylene Glycol) Hydrogels with Localized Transforming Growth Factor β3, Acta biomaterialia, 2019.
  18. Rao, V.V., et al., Rescuing mesenchymal stem cell regenerative properties on hydrogel substrates post serial expansion, Bioengineering & translational medicine, 2019.
  19. Wang, J., et al., Multi-arm PEG-maleimide conjugation intermediate characterization and hydrolysis study by a selective HPLC method, Journal of Pharmaceutical and Biomedical Analysis, 2019, V. 164, P. 452-459.
  20. Carrion, B., et al., The Synergistic Effects of Matrix Stiffness and Composition on the Response of Chondroprogenitor Cells in a 3D Precondensation Microenvironment, Advanced healthcare materials, 2016.
  21. Brown, T.E., et al., Photopolymerized dynamic hydrogels with tunable viscoelastic properties through thioester exchange, Biomaterials, 2018.
  22. Kaphle, P., et al., The mechanical and pharmacological regulation of glioblastoma cell migration in 3D matrices, Journal of cellular physiology, 2019, 234(4):3948-60.
  23. Buwalda, S.J., et al., Ultrafast in situ forming poly (ethylene glycol)-poly (amido amine) hydrogels with tunable drug release properties via controllable degradation rates, European Journal of Pharmaceutics and Biopharmaceutics, 2019, 139:232-9.
  24. Atallah, P., et al., Charge-tuning of glycosaminoglycan-based hydrogels to program cytokine sequestration, Faraday Discussions, 2019.
  25. Dai, J., et al., Modifying Decellularized Aortic Valve Scaffolds with Stromal Cell-derived Factor-1α Loaded Proteolytically Degradable Hydrogel for Recellularization and Remolding, Acta biomaterialia, 2019.
  26. Tunn, I., et al., Bioinspired Histidine–Zn2+ Coordination for Tuning the Mechanical Properties of Self-Healing Coiled Coil Cross-Linked Hydrogels, Biomimetics, 2019, 4(1):25.
  27. Li, H., et al., Synthesis of thiol-terminated PEG-functionalized POSS cross-linkers and fabrication of high-strength and hydrolytic degradable hybrid hydrogels in aqueous phase, European Polymer Journal, 2019, 116:74-83.
  28. Rane, A.A., Understanding mechanisms by which injectable biomaterials affect cardiac function postmyocardial infarction, UC San Diego, 2012.
  29. Maisonneuve, B. G. C., et al., Effects of Synthetic Biomacromolecule Addition on the Flow Behavior of Concentrated Mesenchymal Cell Suspensions, Biomacromolecules 2015, 16(1): 275-283.
  30. Dai, L., et al., Self-assembled targeted folate-conjugated eight-arm-polyethylene glycol–betulinic acid nanoparticles for co-delivery of anticancer drugs, J. Mater. Chem. B, 2015, 3, 3754-3766.
  31. Liu, Ke-Feng, et al., Design, synthesis and in vivo antitumor efficacy of novel eight-arm-polyethylene glycol–pterostilbene prodrugs, RSC Adv., 2015, 5, 51592-51599.
  32. Manzoli, V., et al., Immunoisolation of murine islet allografts in vascularized sites through conformal coating with polyethylene glycol, American Journal of Transplantation, 2018, 18(3):590-603.
  33. Wei, Z., et al., 3D Printing of PEG Hydrogel Scaffolds Using Novel Low Toxicity Photoinitiator, 2018, Society for Biomaterials Meeting poster presentation.
  34. Rao, V.V., et al., Rescuing mesenchymal stem cell regenerative properties on hydrogel substrates post serial expansion, Bioengineering & translational medicine, 2019.
  35. Nguyen, M.K., et al., RNA interfering molecule delivery from in situ forming biodegradable hydrogels for enhancement of bone formation in rat calvarial bone defects, Acta Biomaterialia, V. 75, 2018, P. 105-114.
  36. de Rutte, J.M., et al., Scalable High‐Throughput Production of Modular Microgels for In Situ Assembly of Microporous Tissue Scaffolds, Advanced Functional Materials, 2019.
  37. Manzoli, V., et al., Immunoisolation of murine islet allografts in vascularized sites through conformal coating with polyethylene glycol, American Journal of Transplantation, 2018, 18(3):590-603.
  38. Tomaszewski, C.E., et al., Adipose-derived stem cell-secreted factors promote early stage follicle development in a biomimetic matrix, Biomaterials science, 2019.
  39. Giorgi, M.E., et al., Improved bioavailability of inhibitors of Trypanosoma cruzi trans-sialidase: PEGylation of lactose analogs with multiarm polyethyleneglycol. Glycobiology, 2012, 22(10): p. 1363-1373.
  40. Tong, X. et al, Engineering interpenetrating network hydrogels as biomimetic cell niche with independently tunable biochemical and mechanical properties, Biomaterials 35, 2014, p: 1807-1815
  41. Dietrich, M., et al., Guiding 3D cell migration in deformed synthetic hydrogel microstructures, Soft matter, 2018, 14(15), p.2816-2826.
  42. Shukla, V., et al., Cellular Mechanics of Primary Human Cervical Fibroblasts: Influence of Progesterone and a Pro-inflammatory Cytokine, Annals of biomedical engineering, 2018, 46(1), p.197-207.
  43. Khang, A., et al., Quantifying heart valve interstitial cell contractile state using highly tunable poly(ethylene glycol) hydrogels, Acta Biomaterialia, 2019, 96, p. 354-367.
  44. Rutz, A. L., et al., Employing PEG crosslinkers to optimize cell viability in gel phase bioinks and tailor post printing mechanical properties, Acta Biomaterialia, 2019.
  45. Ju, Y., et al., Engineered Metal-Phenolic Capsules Show Tunable Targeted Delivery to Cancer Cells. Biomacromolecules, 2016.
  46. Dumont, C.M., et al., Aligned hydrogel tubes guide regeneration following spinal cord injury, Acta Biomaterialia, 2019.

Founded in 2001 by experts in PEG synthesis and PEGylation, JenKem Technology specializes exclusively in the development and manufacturing of high quality polyethylene glycol (PEG) products and derivatives, and related custom synthesis and PEGylation services. JenKem Technology is ISO 9001 and ISO 13485 certified, and adheres to ICH Q7A guidelines for GMP manufacture. The production of JenKem® PEGs is back-integrated to in-house polymerization from ethylene oxide, enabling facile traceability for regulated customers. JenKem Technology caters to the PEGylation needs of the pharmaceutical, biotechnology, medical device and diagnostics, and emerging chemical specialty markets, from laboratory scale through large commercial scale.