/Activated PEGs for C-Terminal PEGylation

Activated PEGs for C-Terminal PEGylation

Activated PEGs for C-Terminal PEGylation 2018-06-12T15:37:43+00:00

RnD JenKemJenKem Technology provides high quality polyethylene glycol (PEG) derivatives for C-terminal PEGylation with high purity and low polydispersity, from non-GMP laboratory scale, to large commercial scale in GMP and non-GMP grade.

Amine functionalized PEGs are the most utilized PEG reagents for C-terminus PEGylation proteins and peptides. PEGylation of carboxyl groups with PEG amines requires the presence of coupling agents, such as water-soluble EDC, with or without NHS, for aqueous PEGylation at pH 7.2 [1-4]; or water-insoluble DCC, for non-aqueous carboxyl PEGylation reactions [5].

JenKem Technology manufactures linear and branched multi-arm amine functionalized PEGs, either homofunctional and heterobifunctional. The sterically bulky structure of JenKem Technology’s proprietary Y-shape branched PEG derivatives, consisting of two linear methoxy PEG chains attached to a central core with an active amine group, may help to reduce the number of attachment sites to a protein molecule.

Activated PEG products for C-terminal PEGylation with molecular weights, branching, and functional groups not listed in our online catalog may be available by custom synthesis. Please inquire at tech@jenkemusa.com about pricing and availability of custom PEGs for Carboxyl PEGylation. For global distribution, please visit link. To order directly from JenKem Technology:

Y-shape PEG Amine
≥ 95% Y-shape PEG Amine, more reactive towards acylating agents than the hydroxyl group; readily undergoes reductive amination reactions. JenKem proprietary Y-shape PEGs are more selective, due to their sterically bulky structure. [6]
Methoxy PEG Amine
≥ 95% Methoxy PEG Amine. Attaches via stable linkages, such as amide, urethane, urea, secondary amine; the HCl salt form provides stability for the solid form of M-PEG-NH2 [7, 19, 22-26]
≥ 95% Monodisperse Methoxy PEG5 Amine and Methoxy PEG6 Amine. Attaches via stable linkages, such as amide, urethane, urea, secondary amine. JenKem Technology’s monodisperse PEG products are produced via very reproducible chemical reactions and lack the polydispersity of traditional PEG polymers.
Homobifunctional Amine PEGs
≥ 95% Amine PEG Amine (PEG diamine). PEG Crosslinker, attaches via stable linkages, such as amide, urethane, urea, secondary amines. [8-18]
≥ 95% Monodisperse Amine PEG8 Amine or Amine PEG12 Amine (Discrete PEG bisamine). PEG Crosslinker, attaches via stable linkages, such as amide, urethane, urea, secondary amines. JenKem Technology’s monodisperse PEG products are produced via very reproducible chemical reactions and lack the polydispersity of traditional PEG polymers. [20, 21]
Heterobifunctional Amine Functionalized PEGs:
Multiarm Homofunctional PEGs Functionalized with Amine:
Multiarm Heterobifunctional PEGs Functionalized with Amine and Protected Amine:


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Founded in 2001 by recognized experts in PEG synthesis and PEGylation, JenKem Technology specializes exclusively in the development and manufacturing of high quality polyethylene glycol (PEG) products and derivatives, and related custom synthesis and PEGylation services. JenKem Technology is ISO 9001 and ISO 13485 certified, and adheres to ICH Q7A guidelines for GMP manufacture. The production of JenKem® PEGs is back-integrated to on-site manufacturing from ethylene oxide, enabling facile traceability for GMP regulated customers. JenKem Technology caters to the PEGylation needs of the pharmaceutical, biotechnology, medical device and diagnostics, and emerging chemical specialty markets, from laboratory scale through large commercial scale.