3D Printing

Novel low toxicity photoinitiator M-QTX was launched at the 2018 Society for Biomaterials meeting in poster presentation: “3D Printing of PEG Hydrogel Scaffolds Using Novel Low Toxicity Photoinitiator” [1].

With the introduction of the concept of biological manufacturing, the application of 3D printing technology in tissue engineering and cell culture is a relevant topic. 3D printing biomaterials require biocompatibility, porosity, biodegradability and good adhesion [2]. PEG hydrogels meet these specifications, and in particular PEG acrylate (PEG-ACLT) derivatives are widely applied in hydrogel formation via photopolymerization [3. 4]. Currently, small molecule photoinitiators have a certain level of cytotoxicity, and low water solubility. We have developed a novel photoinitiator (M-QTX) that exhibits good water solubility and low cytotoxicity. Using this photoinitiator, PEG-ACLT hydrogels can be 3D printed through stereolithography into well-defined transparent three-dimensional stereograms with low toxicity and good biocompatibility.

3D printed PEG hydrogel. Precursor solution of JenKem® 8ARM(TP)-ACLT-10K, and photo-initiator M-QTX in water.

M-QTX, a novel photoinitiator with good biocompatibility, high photoinitiation efficiency and good thermal stability was developed for 3D printing of well-defined hydrogel stereograms using PEG-ACLT. Photo-crosslinkable 8ARM(TP)-ACLT-10K derivative was first dissolved in deionized water, followed by the addition of photoinitiator M-QTX. After thorough mixing, the polymer precursor solution was held for two hours protected from light until the bubbles disappeared. Well defined 3D printed hydrogels were then  formed from the photopolymer solution based on Stereolithography using a Formlabs 3D printer-Form 2. The resulting 3D PEG hydrogel scaffold was transparent with a well-defined shape. This hydrogel scaffold with low cytotoxicity and good biocompatibility is suitable for 3D cell culture, tissue engineering, and organ manufacturing.

Suggested JenKem® PEG Products for 3D Printing:

Item Code Item Number
A3247 M-QTX-2000
A3246 M-QTX-3500
A9073 8ARM(TP)-PEG5000-b-PCL1000-ACLT
A9074 8ARM(TP)-PEG10K-b-PCL2000-ACLT
A9075 8ARM(TP)-PEG5000-b-PLA1000-ACLT
A9076 8ARM(TP)-PEG10K-b-PLA2000-ACLT
A8062 8ARM-ACLT-5000
A10055 8ARM(TP)-ACLT-5000
A8063 8ARM-MEACLT-5000
A10056 8ARM(TP)-MEACLT-5000
A8064 8ARM-MEACLT-10K
A10057 8ARM(TP)-MEACLT-10K
A8002 8ARM-ACLT-10K
A10021 8ARM(TP)-ACLT-10K

References:

1. Wei, Z., et al., 3D Printing of PEG Hydrogel Scaffolds Using Novel Low Toxicity Photoinitiator, 2018 Society for Biomaterials poster presentation.

2. Seck, T. M., Melchels, F. P. W., Feijen, J., Grijpma,D. W., J Controlled Release. 2010; 148: 34-41.

3. Arcaute, K., Mann, B. K., Wicker, R. B., Tissue Engineering, Part C. 2011; 17: 27-38.

4. Hockaday, L. A., Colangelo, N. W., Cheung, P. Y. C., et al., Biofabrication. 2012; 4: 035005.

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