Branched Y-Shape PEG Derivatives

JenKem Technology’s patented products, “Y-shaped PEG” derivatives, also known as branched PEG derivatives, contain two linear methoxy PEG chains attached to a central active core. The sterically bulky structure of these “Y-shaped” PEG derivatives facilitates the single point attachment of a molecule. JenKem Technology provides branched PEG derivatives for amine pegylation (Y-NHS-40K), thiol pegylation (Y-MAL-40K), and N-terminal PEGylation (Y-AALD-40K and Y-PALD-40K) [1]. JenKem’s “Y-shaped PEG” derivatives are chemically purified during synthesis and are essentially free of activated bifunctional PEG side products. The typical structure of “Y-shaped” branched PEG is as the following:

ypeg

JenKem Technology Y-Shaped PEGs with molecular weights and functional groups not listed in our online catalog, may be available by custom synthesis. Please inquire at tech@jenkemusa.com about pricing and availability.

PEG PRODUCT SUBSTITUTION REACTIVITY DETAILS
Y-NHS-40K ≥95% Y-shaped NHS PEG ester. Reactive PEG for the amino group of lysine(s), or N-terminal amine [2]; reaction completed in ≤1hr at pH 7-8
Y-COOH-40K ≥95% Y-shaped Carboxyl PEG suitable for amine PEGylation
Y-MAL-40K ≥95% Y-shaped Maleimide PEG. Thiol reactive PEG [3, 4] ; reacts at pH 5.0-6.5
Y-AALD-40K ≥95% Y-shaped Acetaldehyde PEG. N-terminal amine reactive PEG in the presence of a reducing reagent; less reactive but more selective compared to linear PEG aldehydes; reacts at pH 5-8
Y-PALD-40K ≥95% Y-shaped Propion Aldehyde PEG. Reactive PEG for N-terminal amine in the presence of a reducing reagent; less selective but more reactive compared with Y-AALD-40K; reacts at pH 5-8
Y-NH2-40K ≥95% Y-shaped Amine PEG, more reactive towards acylating agents than the hydroxyl group; readily undergoes reductive amination reactions [5]
Y-FITC-40K ≥90% Y-shape Fluorescein PEG, suitable for fluorescence monitoring of the PEGylation reaction with Y-shaped PEGs
Y-ALKYNE-40K ≥95% Y-shaped Alkyne PEG is a convenient click PEG reagent for reaction with azide groups
  1. Hutanu, D., et al., Recent Applications of Polyethylene Glycols (PEGs) and PEG Derivatives. Mod Chem appl 2014. 2(132).
  2. Ashokan, A., et al., Multifunctional calcium phosphate nano-contrast agent for combined nuclear, magnetic and near-infrared in vivo imaging. Biomaterials, 2013. 34(29): p. 7143-7157.
  3. Park, J.-B., et al., PEGylation of bacterial cocaine esterase for protection against protease digestion and immunogenicity. Journal of Controlled Release, 2010. 142(2): p. 174-179.
  4. Resch, G., P. Moreillon, and V.A. Fischetti, PEGylating a bacteriophage endolysin inhibits its bactericidal activity. AMB Express, 2011. 1(29): p. 1-5.
  5. Hoehlig, K., et al., A novel C5a-neutralizing mirror-image (l-)aptamer prevents organ failure and improves survival in experimental sepsis. Mol Ther, 2013. 21(12): p. 2236-46.